A digital microfluidic electrochemical immunoassay.
Identifieur interne : 000261 ( Main/Exploration ); précédent : 000260; suivant : 000262A digital microfluidic electrochemical immunoassay.
Auteurs : RBID : pubmed:24292705Abstract
Digital microfluidics (DMF) has emerged as a popular format for implementing quantitative immunoassays for diagnostic biomarkers. All previous reports of such assays have relied on optical detection; here, we introduce the first digital microfluidic immunoassay relying on electrochemical detection. In this system, an indium tin oxide (ITO) based DMF top plate was modified to include gold sensing electrodes and silver counter/pseudoreference electrodes suitable for in-line amperometric measurements. A thyroid stimulating hormone (TSH) immunoassay procedure was developed relying on magnetic microparticles conjugated with primary antibody (Ab1). Antigen molecules are captured followed by capture of a secondary antibody (Ab2) conjugated with horseradish peroxidase enzyme (HRP). HRP catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) which can be detected amperometrically. The limit of detection of the technique (2.4 μIU mL(-1)) is compatible with clinical applications; moreover, the simplicity and the small size of the detector suggest utility in the future for portable analysis.
DOI: 10.1039/c3lc51063h
PubMed: 24292705
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A digital microfluidic electrochemical immunoassay.</title><author><name sortKey="Shamsi, Mohtashim H" uniqKey="Shamsi M">Mohtashim H Shamsi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Toronto, 80 St George St., Toronto, ON M5S 3H6, Canada. aaron.wheeler@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, University of Toronto, 80 St George St., Toronto, ON M5S 3H6</wicri:regionArea></affiliation></author><author><name sortKey="Choi, Kihwan" uniqKey="Choi K">Kihwan Choi</name></author><author><name sortKey="Ng, Alphonsus H C" uniqKey="Ng A">Alphonsus H C Ng</name></author><author><name sortKey="Wheeler, Aaron R" uniqKey="Wheeler A">Aaron R Wheeler</name></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="doi">10.1039/c3lc51063h</idno><idno type="RBID">pubmed:24292705</idno><idno type="pmid">24292705</idno><idno type="wicri:Area/Main/Corpus">000265</idno><idno type="wicri:Area/Main/Curation">000265</idno><idno type="wicri:Area/Main/Exploration">000261</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Digital microfluidics (DMF) has emerged as a popular format for implementing quantitative immunoassays for diagnostic biomarkers. All previous reports of such assays have relied on optical detection; here, we introduce the first digital microfluidic immunoassay relying on electrochemical detection. In this system, an indium tin oxide (ITO) based DMF top plate was modified to include gold sensing electrodes and silver counter/pseudoreference electrodes suitable for in-line amperometric measurements. A thyroid stimulating hormone (TSH) immunoassay procedure was developed relying on magnetic microparticles conjugated with primary antibody (Ab1). Antigen molecules are captured followed by capture of a secondary antibody (Ab2) conjugated with horseradish peroxidase enzyme (HRP). HRP catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) which can be detected amperometrically. The limit of detection of the technique (2.4 μIU mL(-1)) is compatible with clinical applications; moreover, the simplicity and the small size of the detector suggest utility in the future for portable analysis.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24292705</PMID><DateCreated><Year>2013</Year><Month>12</Month><Day>23</Day></DateCreated><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1473-0189</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Feb</Month><Day>7</Day></PubDate></JournalIssue><Title>Lab on a chip</Title><ISOAbbreviation>Lab Chip</ISOAbbreviation></Journal><ArticleTitle>A digital microfluidic electrochemical immunoassay.</ArticleTitle><Pagination><MedlinePgn>547-54</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c3lc51063h</ELocationID><Abstract><AbstractText>Digital microfluidics (DMF) has emerged as a popular format for implementing quantitative immunoassays for diagnostic biomarkers. All previous reports of such assays have relied on optical detection; here, we introduce the first digital microfluidic immunoassay relying on electrochemical detection. In this system, an indium tin oxide (ITO) based DMF top plate was modified to include gold sensing electrodes and silver counter/pseudoreference electrodes suitable for in-line amperometric measurements. A thyroid stimulating hormone (TSH) immunoassay procedure was developed relying on magnetic microparticles conjugated with primary antibody (Ab1). Antigen molecules are captured followed by capture of a secondary antibody (Ab2) conjugated with horseradish peroxidase enzyme (HRP). HRP catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) which can be detected amperometrically. The limit of detection of the technique (2.4 μIU mL(-1)) is compatible with clinical applications; moreover, the simplicity and the small size of the detector suggest utility in the future for portable analysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shamsi</LastName><ForeName>Mohtashim H</ForeName><Initials>MH</Initials><Affiliation>Department of Chemistry, University of Toronto, 80 St George St., Toronto, ON M5S 3H6, Canada. aaron.wheeler@utoronto.ca.</Affiliation></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Kihwan</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>Alphonsus H C</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Wheeler</LastName><ForeName>Aaron R</ForeName><Initials>AR</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Lab Chip</MedlineTA><NlmUniqueID>101128948</NlmUniqueID><ISSNLinking>1473-0189</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1039/c3lc51063h</ArticleId><ArticleId IdType="pubmed">24292705</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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